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人抗甲型肝炎病毒IgG抗體酶免試劑盒,(anti-HAV)ELISA檢測試劑盒 ,英文名:anti-HAV ELISA Kit ,英文縮寫:anti-HAV ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗甲型肝炎病毒IgG抗體ELISA檢測試劑盒,anti-HAV 檢測試劑盒, anti-HAV 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗甲型肝炎病毒IgG抗體酶免試劑盒,(anti-HAV)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重
血紅蛋白(HB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hemoglobin (HB)48T/96TY09435Homo sapiens (Human)
垂體腺苷酸環(huán)化酶激活肽受體(PACAPR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pituitary Adenylate Cyclase Activating Polypeptide Receptor 1 (PACAPR)48T/96TY09436Homo sapiens (Human)
腺苷酸環(huán)化酶3(ADCY3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 3 (ADCY3)48T/96TY09437Homo sapiens (Human)
交替氧化酶(AOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alternative Oxidase (AOX)48T/96TY09438Spinacia oleracea (Spinach)
腺苷酸環(huán)化酶4(ADCY4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 4 (ADCY4)48T/96TY09439Homo sapiens (Human)
腺苷酸環(huán)化酶5(ADCY5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 5 (ADCY5)48T/96TY09440Homo sapiens (Human)
葡萄糖苷酸酶β(GUSβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucuronidase Beta (GUSb)48T/96TY09441Spinacia oleracea (Spinach)
蔗糖磷酸合酶(SPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sucrose Phosphate Synthase (SPS)48T/96TY09442Spinacia oleracea (Spinach)
腺苷酸環(huán)化酶6(ADCY6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 6 (ADCY6)48T/96TY09443Homo sapiens (Human)
腺苷酸環(huán)化酶7(ADCY7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 7 (ADCY7)48T/96TY09444Homo sapiens (Human)
腺苷酸環(huán)化酶8(ADCY8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 8, Brain (ADCY8)48T/96TY09445Homo sapiens (Human)
210kDa核孔蛋白(NUP210)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nucleoporin 210kDa (NUP210)48T/96TY09446Homo sapiens (Human)
早期B-細(xì)胞因子1(EBF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early B-Cell Factor 1 (EBF1)48T/96TY09447Homo sapiens (Human)
早期B-細(xì)胞因子2(EBF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early B-Cell Factor 2 (EBF2)48T/96TY09448Homo sapiens (Human)
β-促脂素(βLPH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Lipotropic Hormone (bLPH)48T/96TY09449Homo sapiens (Human)
干擾素α/β受體(IFNα/βR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Alpha/Beta Receptor 1 (IFNa/bR1)48T/96TY09450Homo sapiens (Human)
腺苷酸環(huán)化酶9(ADCY9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 9 (ADCY9)48T/96TY09451Homo sapiens (Human)
腺苷酸環(huán)化酶10(ADCY10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Adenylate Cyclase 10, Soluble (ADCY10)48T/96TY09452Homo sapiens (Human)
多巴胺β羥化酶(DβH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Beta Hydroxylase (DbH)48T/96TY09453Homo sapiens (Human)
絲裂原激活蛋白激酶7(MAPK7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase 7 (MAPK7)48T/96TY09454Homo sapiens (Human)
肌特異性烯醇化酶(MSE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Muscle Specific (MSE)48T/96TY09455Homo sapiens (Human)
蛋白酶3(PR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proteinase 3 (PR3)48T/96TY09456Rattus norvegicus (Rat)
絲裂原激活蛋白激酶11(MAPK11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase 11 (MAPK11)48T/96TY09457Homo sapiens (Human)
巨噬細(xì)胞幼紅細(xì)胞黏附蛋白(MAEA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Macrophage Erythroblast Attacher (MAEA)48T/96TY09458Homo sapiens (Human)
酪氨酸羥化酶(TH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tyrosine Hydroxylase (TH)48T/96TY09459Homo sapiens (Human)
葡萄糖氧化酶(GOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Oxidase (GOD)48T/96TY09460Homo sapiens (Human)
趨化因子C-X3-C-基元受體1(CX3CR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Chemokine C-X3-C-Motif Receptor 1 (CX3CR1)48T/96TY09461Homo sapiens (Human)
神經(jīng)元凋亡抑制蛋白(NAIP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuronal Apoptosis Inhibitory Protein (NAIP)48T/96TY09462Homo sapiens (Human)
親環(huán)素D(CYPD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclophilin D (CYPD)48T/96TY09463Homo sapiens (Human)
纖溶酶(Fbn)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibrinolysin (Fbn)48T/96TY09464General
血小板膜糖蛋白Ⅳ(GP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plaet Membrane Glycoprotein IV (GP4)48T/96TY09465Homo sapiens (Human)
殺傷細(xì)胞凝集素樣受體亞家族A成員1(KLRA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Killer Cell Lectin Like Receptor Subfamily A, Member 1 (KLRA1)48T/96TY09466Homo sapiens (Human)
白三烯E4(LTE4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene E4 (LTE4)48T/96TY09467General
前列腺素F2α(PGF2α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin F2 Alpha (PGF2a)48T/96TY09468General
白介素7(IL7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 7 (IL7)48T/96TY09469Mus musculus (Mouse)
Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 2 (TLR2)48T/96TY09470Rattus norvegicus (Rat)
Toll樣受體6(TLR6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 6 (TLR6)48T/96TY09471Rattus norvegicus (Rat)
髓樣前體細(xì)胞抑制因子2(MPIF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myeloid Progenitor Inhibitory Factor 2 (MPIF2)48T/96TY09472Homo sapiens (Human)
多巴胺受體D5(DRD5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D5 (DRD5)48T/96TY09473Homo sapiens (Human)
鈣調(diào)磷酸酶(CaN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calcineurin (CaN)48T/96TY09474Rattus norvegicus (Rat)
胱天蛋白酶12(CASP12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 12 (CASP12)48T/96TY09475Homo sapiens (Human)
熱休克轉(zhuǎn)錄因子4(HSF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Transcription Factor 4 (HSF4)48T/96TY09476Homo sapiens (Human)
丙酮酸脫氫酶激酶同工酶4(PDK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)48T/96TY09477Homo sapiens (Human)
Ⅰ型前膠原氨基端原肽(PⅠNP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)48T/96TY09478Homo sapiens (Human)
維甲酸受體α(RARα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoic Acid Receptor Alpha (RARa)48T/96TY09479Homo sapiens (Human)
磷脂酰甘油(PG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidylglycerol (PG)48T/96TY09480General
磷脂酸(PA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphatidic Acid (PA)48T/96TY09481General
白介素1β(IL1β)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Interleukin 1 Beta (IL1b)48T/96TY09482Chicken (Gallus)
免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Immunoglobulin A (IgA)48T/96TY09483Chicken (Gallus)
信號傳導(dǎo)轉(zhuǎn)錄激活因子6(STAT6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 6 (STAT6)48T/96TY09484Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。